Coding

Part:BBa_K4651011:Design

Designed by: Jason Gao   Group: iGEM23_DNHS-SanDiego-CA   (2023-10-10)


Imports TPA into plasmid


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 992
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 537
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

N/A


Source

Pardo I, Jha RK, Bermel RE, Bratti F, Gaddis M, McIntyre E, Michener W, Neidle EL, Dale T, Beckham GT, Johnson CW. Gene amplification, laboratory evolution, and biosensor screening reveal MucK as a terephthalic acid transporter in Acinetobacter baylyi ADP1. Metab Eng. 2020 Nov;62:260-274. doi: 10.1016/j.ymben.2020.09.009. Epub 2020 Oct 1. PMID: 32979486.

References

Pardo I, Jha RK, Bermel RE, Bratti F, Gaddis M, McIntyre E, Michener W, Neidle EL, Dale T, Beckham GT, Johnson CW. Gene amplification, laboratory evolution, and biosensor screening reveal MucK as a terephthalic acid transporter in Acinetobacter baylyi ADP1. Metab Eng. 2020 Nov;62:260-274. doi: 10.1016/j.ymben.2020.09.009. Epub 2020 Oct 1. PMID: 32979486.